Physical and Chemical Treatment Effects on the Germination of Pear Seeds (Pyrus Communis L.)

(Ziad J. Al-Hussein and Marwa S. Al-Shabib)

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Abstract

Pear (Pyrus communis L.) is one of the important species of fruits that are widely cultivated in Syria. Seed propagation of the pear is an important way to obtain seedlings to be used as grafting assets, but the method of seed propagation is difficult due to the effect of dormancy factors in germination. The research was carried out during 2020 and 2021 to study the effects of stratification for three periods (30–60–90 days) using ascorbic acid at a concentration of 100 mg/L, and potassium nitrate at a concentration of 0.3% for breaking the dormancy of pear seeds. The results showed that the best averages were at stratification for 60 days and gave the percentage of germination as 31%, and the speed of germination at 1.28 seeds/day. Additionally, treatments of seed soaking with ascorbic acid or potassium nitrate (without stratification) contributed to improving the germination percentage to 7.67% and 3.67%, respectively, with significant differences compared with the control (1%). The best results were with stratification treatment for 60 days with ascorbic acid, where the percentage of germination reached 49.67% and the germination speed increased to 3.91 seeds/day, in addition to reducing the period required for germination to 21 days.
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References

Aatla, H. and Srihari, D. (2013). Influence of pre-sowing treatments on germination, growth and vigour of mango cv. Alphonso, Horticultural University, West Godavari (A.P.) India. Theasian Journal of Horticulture, 8(1), 122–5. 
Akram, N.A., Shafiq, F. and Ashraf, M. (2017). Ascorbic acid-a potential oxidantscavenger and its role in plant development and abiotic stress tolerance. Frontiers in Plant Science, 8(613), 1–17.
Al-Douri, I.F. (2007). Tathir Alkibrit Walnitrujin Wahimd Al'uskurbik Fi Alnumui Alkhudrii Walmuhtawaa Almaedinii Li'ashjar Altufaah Alfatyat Sinfi Anna Wa Vista ‘The effect of Sulfur, Nitrogen and Ascorbic Acid on the Vegetative Growth and Mineral Content of Young Apple Trees Anna and Vista’. Master’s Dissertation, University of Mosul, College of Agriculture and Forestry, Nineveh, Iraq. [in Arabic]
Bethke, P.C., Libourel, I.G.L. and Jones, R.L. (2006). Nitric oxide reduces seed dormancy in Arabidopsis. Journal of Experimental Botany, 57(3), 517–26.
Blokhina, O., Virolainen, E. and Fagerstedt, K.V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress. Annals of Botany, 91(2), 179–97.
Çalişkan, O., Mavim, K. and Polat, A. (2012). Influences of presowing treatments on the germination and emergence of fig seeds (Ficus carica L.). Agronomy Maringá, 34(3), 293–7.
Cetinbas, M., and Koyuncu, F. (2006). Improving germination of Prunus avium L. seeds by gibberellic acid, potassium nitrate and thiourea. Hort. Sci. (Prague), 33(3), 119–23.
Dawai, F. and Ismail, H. (2004). Almashatil Wal'iikthar Alkhudari ‘Nurseries and Vegetative Propagation’. Lattakia: Directorate of Books and Publications. [in Arabic].
De Tullio, M.C. and Arrigoni, O. (2003). The ascorbic acid system in seeds: To protect and to serve. Seed Science Research, 13(04), 249–60.
Demirsoy, L., Demirsoy, H., Celikel, G., Macit, I. and Ersoy, B. (2010). Seed treatment with GA3 or stratification enhances emergence of some strawberry tree genotypes. Horticultural Science, 37(1), 34–7.
Dewir, Y.H., El-Mahrouk, M.E.S. and Naidoo, Y. (2011). Effects of some mechanical and chemical treatments on seed germination of 'Sabal palmetto and Thrinax morrisiiPalms. Aust. J. Crop Sc, 5(3), 248–53.
Dolatabadian, A., Mohammad, S. and Sanavy, M. (2008). Effect of the Ascorbic Acid, Pyridoxine and Hydrogen Peroxide treatments on germination, Catalase activity, Protein and Malondialdehyde content of three oil seeds. Hort. Agrobot. Cluj, 36(2), 61–6. 
EL-Refaey, F.A. and EL-Dengawy, E.F.A. (2005). Promotion of seed germination and subsequent seedling growth of Loquat (Eriobotrya japonica, Lindl) by moist-chilling and GA3 applications. Scientia Horticulturae, 105(3), 331–42.
Esechie, H. (1994). Interaction of salinity and temperature on the germination of Sorghum. J. Agron. Crop. Sci, 172(n/a), 194–9. 
FAO. (2021). Statistical Databases. Rome: Food and Agriculture
Organization of the United Nations.
Gashi, B., Abdullai, K., Mata, V. and Kongjika, E. (2012). Effect of gibberellic acid and potassium nitrate on seed germination of the resurrection plants Ramonda serbica and Ramonda nathaliae. African Journal of Biotechnology, 11(20), 4537–42.
Górnik, K., Grzesik, M., Janas, R. and Żurawicz, E. (2018). The effect of Apple seed stratification with growth regulators on breaking the dormancy of seeds, the growth of seedlings and chlorophyll fluorescence. Journal of Horticultural Research, 26(1), 37–44.
Hamama, H. and Murniati, E. (2010). The effect of ascorbic acid treatment on viability and vigor maize (Zea mays L.) seedling under drought stress. Journal of Biosciences, Bogor Agricultural University, 17(3), 105–9.
Irfan, M., Jan, G., Jan, F.G. and Murad, W. (2021). Influence of ascorbic acid against salt stress on the germination, biochemicals and micronutrients concentrations of Solanum melongena (L.). Preprints, 1(273), 1–17. 
Joshi, C., Shekhar, R., Tapan, K. and Nailwal, K. (2016). Effect of gibberellic acid, potassium nitrate and chilling on seed germination response of Apple (Pyrus malus l. cv. red delicious). International Journal of Advanced Research, 4(6), 1141–55.
Lewak, S. (2011). Metabolic control of embryonic dormancy in Apple seed: seven decades of research. Acta Physiologiae Plantarum, 33(1), 1–24.
Lisko, K.A., Aboobucker, S.I., Torres, R. and Lorence, A. (2014). Engineering elevated vitamin C in plants to improve their nutritional content, growth, and tolerance to abiotic stress. Function and Application, n/a(n/a), 110–28.
Manjkhola, S., Dhar, U. and Rawal, R. (2003). Treatments to improve seed germination in Arnebia benthami: An endangered medicinal herb of high-altitude Himalaya. Seed Science and Technology, 31(3), 571–7.
Moradi, D.P., Sharif-zadeh, F. and Janmohammadi, M. (2008). Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). J. Agr. Biol. Sci, 3(3), 22–5.
Mukhawil, J. (2010). Tathir baed almueamalat fi kasr tawr alraahat libudhur baed turuz alkamuthraa alsuwria (alkomuthraa alsuwri) Pyrus syriaca ‘Effect of some treatments on resting phase fracture of seeds of some Syrian Pear (Pyrus syriaca)’. Al-Furat University Journal for Scientific Studies and Research, Basic Sciences Series, 1(3) 102–18. [in Arabic]
Niu, J., Zhao, L., Fan, Y., Shi, S., He, L. and Hui, W. (2019). The effects of ascorbic acid on breaking the seed dormancy of Malus Sieversii. Journal of plant growth regulation, 38(3), 909–18. 
Rajabi, R. and Poustini, K. (2005). Effects of NaCl salinity on seed germination of 30 Wheat (Triticum aestivum L.). Cultivars. SciJ. Agric, 28(n/a), 29–44.
Rajamanickam, C., Anbu, S. and Balakrishnan, K. (2004). Influence of seed treatments on seedling vigour in amla (Emblica officinalis G.). South Indian J. Hort, 52(1), 324–7.
Rawat, J.M.S., Tomar, Y.K. and Rawat, V. (2010). Effect of stratification on seed germination and seedling performance of wild pomegranate. Journal of American Science, 6(5), 97–9.
Suleiman, S. and Omran, L. (2011). Tathir baed alturuq alfizyayiyat walkimyayiyat fi 'iinbat budhur altufaah nawe Golden delicious ‘Effect of some physical and chemical methods on germination of Golden delicious apple seeds’. Tishreen University Journal for Scientific Research and Studies, 33(6), 59–73. [in Arabic]
Syrian Annual Agricultural Statistical Group. (2020). Tawazue Ziraeat 'Ashjar Alfakihah ‘Distributed Cultivation of Fruit Trees’. Damascus: Statistical Office of the Ministry of Agriculture and Agrarian Reform. [in Arabic]
Tipirdamaz, R. and Ver-Gomurgen, A.N. (2000). The effect of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) Salisb. seeds. Turkish Journal of Botany, 24(2), 143–5.