Microscopic evaluation for the protective role of ascorbic acid against lead toxicosis in male rats

(Mostafa M. Hasseeb, Fahad A. Al-Hizab and Mahmoud M. Hamouda.)

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Abstract

The present study was carried out to evaluate the microscopic changes related to the protective effect of supplementation with two levels (2000 and 4000 ppm in drinking water) of ascorbic acid (AA), against lead (Pb) toxicosis (150 ppm in drinking water) in rats. One hundred and eighty adult male Sprague-Dawley rats were used. The concurrent daily administration for each of the two levels of AA with the toxic dose of Pb acetate) was continued allover the 3 months of the experiment after which necropsy and histopathologic examination were done. The histopathologic examination in the first trial for treatment with the low level of AA revealed no or less protective effect where some mild to moderate changes of Pb toxicosis in some organs were seen. The histopathologic examination in the second trial for treatment with the high level of AA revealed an obvious alleviating effect on various body organs. In conclusion for the present study the supplementation with AA resulted in an appreciable improvement in the architecture of body organs, especially at levels of 4000 ppm. Key words: Ascorbic acid; Vitamin C; Lead acetate; Antioxidant; Histopathology; Toxicosis; Lead acetate; Lead poisoning; Plumbism; Rats .
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