Detecting of some Fungicides Ability to Induce Resistant Mutations in Aspergillus amstelodami.

(Fadeya M. Al-Hyaly and Huda W. Hadi)

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Abstract

The erroneous and indiscriminate use of fungicides led to increasing resistance of some of these fungicides to many fungi, which posed serious threats to agricultural production. This study investigated the minimum inhibitory concentration (MIC) of both systemic fungicide (Hexaconazol 10% and Prochloraz 25%) on the occurrence of resistant gene mutations in Aspergillus amstelodami. Seventeen mutations of spontaneous resistance with an average recurrence of 3.95x105 at (MIC) 300 μg / ml of Hexaconazol, and 20 spontaneous resistance mutation with average recurrence of 26x4.105 at (MIC) 1.25 μg / ml of the Prochloraz were used. The study recorded the isolation of 94 hexaconazol induced nitrous acid -resistant strains with an average frequency of 39.388 x105 and 100 mutation induced by nitrous acid with an average recurrence of 41.171x105 resistant to the Prochloraz. Two spontaneous mutants, 7 nitrous-induced mutations were resistant to Hexaconazol and cross resistance to Prochloraz, while 8 spontaneous mutations, 15 mutants induced with nitrous acid showed resistance to Prochloraz and cross resistance to Hexaconazol. We conclude from the above that, A. amstelodami is able to resist the studied fungicides, as well as the presence of cross-resistance between the strains' resistant to fungicides. This reduces these fungicides importance in the fight against fungi and should be taken into account when using these particular fungicides against fungal infections of the plant. Key Word: Cross-resistance, Hexaconazol, Nitrous acid, Prochloraz, Strain.
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