Detection of Alginate alg D gene of Pseudomonas aeruginosa

(Rana M. Abdullah Al-Shwaikh and Abbas Falih Al-arnawtee)

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Abstract

Pseudomonas aeruginosa are opportunistic pathogens that infect humans causing many diseases, which may lead to the death of a person. Alginate are important virulence factors of the bacterium, which provides protection to the bacteria cell layer. One hundred isolates of Pseudomonas aeruginosa were collected during the period between 1/9/2014 to 1/11/2014 from Central Teaching Hospital of Pediatric, Medical city department of teaching laboratories, and Burns Hospital /Medical City, in Baghdad, Iraq. After identification, seventy five isolates were confirmed to be P. aeruginosa including 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood. Lab work was performed in the laboratories of Molecular and Microbiology in Department of Biology / College of Education Ibn- Al Haitham / University of Baghdad. The isolates were identified by culturing on MacConkey agar, Cetrimide agar, Pseudomonas agar, and CHROM agar then identified by performing biochemical tests including oxidase test, catalase test, and were further identified using an API20E system Genotypic detection for virulence genes alginate D of Pseudomonas aeruginosa was performed using PCR. The result revealed that the alginate D gene was present in 56 isolates (74.66 %) of Pseudomonas aeruginosa. The gel electrophoresis showed that the size of alginate D gene was 1310 bp. Seventy five isolates of Pseudomonas aeruginosa had alginate D including 21 isolates from otitis media (75%), 14 isolates from burn infections (60.86%) , 9 isolates from wound infections (90%), 6 isolates from urinary tract infections (75%), and 6 isolates from blood (100%). Key Words: PCR, Pseudomonas aeruginosa, Virulence factor.
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