Scientific Journal Of King Faisal University
Basic and Applied Sciences

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Scientific Journal of King Faisal University / Basic and Applied Sciences

Effects of Brassica Rapa on Dyslipidaemia and Oxidative Damage in Rats Consuming a Hyperlipidic Diet

(Fatima Zohra El kadi, Karima Ould Yerou and Hadj Mostefa Khelladi)

Abstract

This research investigated the influence of a hyperlipidic diet in Wistar rats and the therapeutic effect of Brassica rapa powder (BrP). A hyperlipidic diet was administered followed by oral supplementation of BrP (5%) for 28 days. The BrP supplementation caused a decrease in weight (- 41%), glycaemia (-12%), creatinemia (-51%), aspartate aminotransferase (AST) level (-80%), and cholesterolaemia (-25%) associated with an increase in HDLc level (+59%). However, the hepatic lipid profile showed a decrease in phospholipids (PL) (-59%) and total cholesterol (TC) (-67%). In addition, oxidative stress assessment showed a decrease in the serum (-22%), renal (-30%) and adipose (-8%) levels of TBARS. Furthermore, BrP supplementation boosted glutathione (GSH) protective activity in adipose tissue with a maximum of +49%. In conclusion, Brassica rapa enhances the serum and tissue balance of metabolic and antioxidant status.
KEYWORDS
Antioxidant, biomarkers, fat, in vivo, redox status, turnip

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References

Abo-youssef, A.M. and Mohammed, R. (2013). Effects of Brassica rapa on fructose-induced metabolic syndrome in rats: a comparative study. International Journal of Pharmaceutical Sciences Review and Research, 21(1), 1–5.
Al-Snafi, A.E. (2015). Therapeutic properties of medicinal plants: a review of their detoxification capacity and protective effects (part 1). Asian Journal of Pharmaceutical Science and Technology, 5(4), 257–270.
Amrita-Bhowmik, A.B., Khan, L.A., Masfida Akhter, M.A. and Begum Rokeya, B.R. (2009). Studies on the antidiabetic effects of Mangifera indica stem-barks and leaves on nondiabetic, type 1 and type 2 diabetic model rats. Bangladesh Journal Pharmacol, 4(2), 110–114. DOI:10.3329/bjp.v4i2.2488
An, S., Han, J.I., Kim, M.J., Park, J.S., Han, J.M., Baek, N.I. and Jeong, T.S. (2010). Ethanolic extracts of Brassica campestris spp. rapa roots prevent high-fat diet-induced obesity via β 3-adrenergic regulation of white adipocyte lipolytic activity. Journal of Medicinal Food, 13(2), 406–414.DOI:10.1089/jmf.2009.1295
Burtis, C.A. and Ashwood, E.R. (1994). Tietz textbook of clinical chemistry. Philadelphia, USA: Amer Assn for Clinical Chemistry.
Chen, G.C., Koh, W.P., Yuan, J.M., Qin, L.Q. and van Dam, R.M. (2018). Green leafy and cruciferous vegetable consumption and risk of type 2 diabetes: results from the Singapore Chinese Health Study and meta-analysis. British Journal of Nutrition, 119(9), 1057–1067. DOI: 10.1017/S0007114518000119.
Delsal, J.L. (1944). New method of extraction of serum lipids by methylal. Application to micro-estimation of total cholesterol, phospho-aminolipins and proteins. Bulletin de la Société de chimie Biologique, 26(n/a), 99–105.
Dias, J.S. (2019). Nutritional quality and effect on disease prevention of vegetables. In: Nutrition in health and disease-our challenges now and forthcoming time. London, UK: IntechOpen.
Fawcett, J. and Scott, J. (1960). A rapid and precise method for the determination of urea. Journal of clinical pathology, 13(2), 156–159. DOI: 10.1136/jcp.13.2.156
Feillet-Coudray, C., Sutra, T., Fouret, G., Ramos, J., Wrutniak-Cabello, C., Cabello, G. and Coudray, C. (2009). Oxidative stress in rats fed a high-fat high-sucrose diet and preventive effect of polyphenols: Involvement of mitochondrial and NAD (P) H oxidase systems. Free Radical Biology and Medicine, 46(5), 624–632. DOI: 10.1016/j.freeradbiomed.2008.11.020
Fresco, P., Borges, F., Marques, M.P.M. and Diniz, C. (2010). The anticancer properties of dietary polyphenols and its relation with apoptosis. Current pharmaceutical design, 16(1), 114–134. DOI: 10.2174/138161210789941856
Friedewald, W.T., Levy, R.I. and Fredrickson, D.S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry, 18(6), 499–502. DOI :10.1093/clinchem/18.6.499
Ghasemi, A., Khalifi, S. and Jedi, S. (2014). Streptozotocin-nicotinamide-induced rat model of type 2 diabetes. Acta Physiologica Hungarica, 101(4), 408–420. DOI:10.1556/APhysiol.101.2014.4.2
Hattori, M., Nikolic-Paterson, D.J., Miyazaki, K., Isbel, N.M., Lan, H.Y., Atkins, R.C. and Ito, K. (1999). Mechanisms of glomerular macrophage infiltration in lipid-induced renal injury. Kidney International, 56(n/a), S47–S50. DOI: 10.1046/j.1523-1755.1999.07112.x
Jung, U.J., Baek, N.I., Chung, H.G., Bang, M.H., Jeong, T.S., Lee, K.T. and Choi, M.S. (2008). Effects of the ethanol extract of the roots of Brassica rapa on glucose and lipid metabolism in C57BL/KsJ-db/db mice. Clinical Nutrition, 27(1), 158–167. DOI: 10.1016/j.clnu.2007.09.009
Kapusta-Duch, J., Kopec, A., Piatkowska, E., Borczak, B. and Leszczynska, T. (2012). The beneficial effects of Brassica vegetables on human health. Roczniki Państwowego Zakładu Higieny, 63(4), 389–95.
Kim, E.J., Kim, B.H., Seo, H.S., Lee, Y.J., Kim, H.H., Son, H.H. and Choi, M.H. (2014). Cholesterol-induced non-alcoholic fatty liver disease and atherosclerosis aggravated by systemic inflammation. PloS one, 9(6), e97841. DOI:10.1371/journal.pone.0097841
Kim, Y.H., Kim, Y.W., Oh, Y.J., Back, N.I., Chung, S.A., Chung, H.G. and Lee, K.T. (2006). Protective effect of the ethanol extract of the roots of Brassica rapa on cisplatin-induced nephrotoxicity in LLC-PK1 cells and rats. Biological and Pharmaceutical Bulletin, 29(12), 2436–2441. DOI: 10.1248/bpb.29.2436
Lim, S.S., Vos, T., Flaxman, A.D., Danaei, G., Shibuya, K., Adair-Rohani, H. and Pelizzari, P.M. (2012). A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study. The lancet, 380(9859), 2224–2260. DOI: 10.1016/S0140-6736(12)61766-8.
Lopes-Virella, M.F., Stone, P., Ellis, S. and Colwell, J.A. (1977). Cholesterol determination in high-density lipoproteins separated by three different methods. Clinical chemistry, 23(5), 882–884. DOI : 10.1093/clinchem/23.5.882
López-Chillón, M.T., Carazo-Díaz, C., Prieto-Merino, D., Zafrilla, P., Moreno, D.A. and Villaño, D. (2019). Effects of long-term consumption of broccoli sprouts on inflammatory markers in overweight subjects. Clinical Nutrition, 38(2), 745–752. DOI :10.1016/j. clnu.2018.03.006
Micha, R. and Mozaffarian, D. (2010). Saturated fat and cardiometabolic risk factors, coronary heart disease, stroke, and diabetes: a fresh look at the evidence. Lipids, 45(n/a), 893–905. DOI:10.1007/s11745-010-3393-4
Okawa, H., Ohishi, N. and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95(2), 351–358. DOI:10.1016/0003-2697(79)90738-3
Quintanilha, A.T., Thomas, D.D. and Swanson, M. (1982). Protein-lipid interactions within purified and reconstituted cytochrome c reductase and oxidase. Biophysical journal, 37(1), 68. DOI: 10.1016%2FS0006-3495(82)84603-1
Raiola, A., Errico, A., Petruk, G., Monti, D.M., Barone, A. and Rigano, M.M. (2017). Bioactive compounds in Brassicaceae vegetables with a role in the prevention of chronic diseases. Molecules, 23(1), 15. DOI:10.3390/molecules23010015
Ramirez, D., Abellán-Victorio, A., Beretta, V., Camargo, A. and Moreno, D.A. (2020). Functional ingredients from Brassicaceae species: Overview and perspectives. International journal of molecular sciences, 21(6), 1998. DOI :10.3390/ijms21061998
Reitman, S. and Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology, 28(1), 56–63. DOI: 10.1093/ajcp/28.1.56
Richmond, W. (1973). Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clinical chemistry, 19(12), 1350–1356. DOI: 10.1093/clinchem/19.12.1350
Šamec, D. and Salopek-Sondi, B. (2019). Cruciferous (brassicaceae) vegetables. In: Nonvitamin and nonmineral nutritional supplements. San Diego, California: Academic Press.
Schirmeister, J., Willmann, H. and Kiefer, H. (1964). Plasmakreatinin als grober Indikator der Nierenfunktion. DMW-Deutsche Medizinische Wochenschrift, 89(21), 1018–1023. DOI: 10.1055/s-0028-1111251
Sedlak, J. and Lindsay, R.H. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical biochemistry, 25(n/a), 192–205.
Soliman, A.M., Mohamed, A.S. and Marie, M.A.S. (2016). Effect of echinochrome on body weight, musculoskeletal system and lipid profile of male diabetic rats. Austin J Endocrinol Diabetes, 3(2), 1045.
Thomas-Charles, C. and Fennell, H. (2019). Anti-prostate cancer activity of plant-derived bioactive compounds: A review. Current Molecular Biology Reports, 5(n/a), 140–151. DOI:10.1007/s4061 0-019-00123-x
Tietz, N.W., Finley, P., Pruden, E. and Amerson, A. (1990). Clinical guide to laboratory tests Saunders 2nd Edition. Philadelphia, USA: WB Company.
Yerou, K.O., EL KADI, F.Z., Kanoun, K., Khelladi, H.M., Benzahia, H. and Bekhti, S. (2022). Preliminary phytochemical investigation and antioxidant potential of various extracts of dietary turnip (Brassica rapa L.). Food and Environment Safety Journal, 21(1), 94–106. DOI: 10.4316/fens.2022.010