Enhancement and Optimization of cellulose production by Gluconacetobacter xylinus N2

(Diaa Nour and Hamid Majid Hussein Algelaoa and Hamid Majid Jassem)

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Abstract

To enhance the ability of G. xylinus N2 for cellulose production, this isolate was subjected to random mutagenesis using UV radiation, two enhanced producer mutants (G. xylinus N2_87 and G. xylinus N2_90) were obtained and the productivity of cellulose in their culture filtrate was 6.3 and 6.9 g/L (increased by about 26% and 38%) respectively. Optimum conditions for cellulose production by G. xylinus N2_90 were growing this bacterium in production medium (HS medium) containing date syrup (2%) as a sole source of carbon and energy, yeast extract (2%) as a nitrogen source, in an initial pH 6.5 and incubation at 30176;C for one week. Under these conditions, cellulose dry weight in culture filtrate of G. xylinus N2_90 was 8.5 g/L. Key Words: Cellulose, Gluconacetobacter xylinus, Mutagenesis, Optimum conditions.
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