The Chemical Effect of Some Irrigation Solutions Used in RCT on the Structure of Radicular Dentin by Using XRF Technique

(Margana Kotaivani and kinda Leos)

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Abstract

Saving the chemical structure of radicular dentine while using the irrigation solutions in the root canals treatment (RCT) is very important. The aim of this study was to evaluate ions contents of radicular dentin after treatment with several endodontic irrigation solutions as Sodium Hypochlorite NaOCl 5.25%, Ethylene diamine tetra acetic acid (EDTA), a Mixture of tetracycline isomer and a detergent (Bio pure MTAD) for 20 minutes on the radicular dentin. Fifty intact freshly extracted single rooted mandibular anterior human teeth were taken and stored in normal saline solution until used. The crowns of the teeth were removed at the cemento-enamel junction .pulp tissues were removed and the teeth were randomly divided into five groups(n=10). Group I teeth were treated with Distilled water for 20 minutes. Group II teeth were treated with NaOCl 5.25% for 20 minutes. Group III teeth were treated with NaOCl 5.25% and 17% EDTA in succession for 20 minutes. Group IV teeth were treated with distilled water for 15 minutes and BioPure MTAD for 5 minutes as instructed by the company. Group V teeth were treated with NaOCl 1.3% and Bio Pure MTAD for 20 minutes. Dentin Chips were obtained using Gattes-Glidden burs .the concentration of Calcium, Zinc, Iron, and Strontium in each Specimens was analyzed using XRF technique. Changes in the concentrations of the chemical elements were recorded. Results from this research indicate that endodontic irrigation solutions have an effect on the concentration of minerals in the radicular dentin. There was a statistically decrease with all irrigation solutions except for Strontium (P<0.05). Key Words: mineral content, Radicular dentin, XRF Technique, RCT, Irrigation solutions.
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