Line Regeneration from Stressed Callus in Three Potato (Solanum tuberosum L) Varieties and Evaluation of Their Salinity Tolerance

(Rima Massa, Fahed Al-Biski, Khalil Al-Maarri)

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Abstract

Salt stress is one of the major abiotic stresses in Syria that limits potato production. This research was carried out in the laboratories of National Commission for Biotechnology and Faculty of Agriculture (Damascus). It aimed to study the effect of salt stress on the changes in some growth parameters (plant length and diameter, leaf area, number of leaves, number and length of roots, plus wet and dry weight) in regenerated plantlets of stressed callus of three In vitro Potato varieties (Taurus, Safari and Sfinja). The highest regeneration percentage was recorded in Sfinja cultivar (37.50 %) , Safari (31.25 %) that were treated with MS media containing 1.5 mgl-1 BAP+ 25 mgl-1 adenine sulphate, while it was (34.33 %) in Taurus cultivar with MS media containing 1mgl-1 BAP + 0.5 mgl-1 IAA. The Line (S9) was the best line of the Sfinja cultivar in plant height and number of roots, while the line (SF5) was the most superior line of the Safari cultivar in plant diameter, leaf area, and roots number). The line (T1) exceeded the other lines of the Taurus cultivar in root length, wet, and dry weights. The research suggests continuing work to study the degree of genetic variance between the studied varieties and their regenerated genotypes using ISSR, and Study their behavior in vivo under the salt stress. Key Words: Callus, Potato, Regeneration, Salinity stress.
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