Characterization of Candidate Seed Orf Viruses to be Used as Vaccine in Sheep and Goats in Saudi Arabia

(Fadhel bin Mohammed Taher Hawsawi)

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Abstract

According to the Office International Des Epizooties (OIE) standards for production of poxviruses vaccines, it is a pre-requisite to perform characterization of the candidate seed virus before its incorporation as a seed for the particular vaccine. In the present study, two candidate field orf viruses were subjected to characterization according to the OIE standards. Treatment of the viruses with Chloroform and diethyl ether has resulted in dropping of the titre of each virus by 2 log10 and 1.3 log10 respectively. Sensitivity to trypsin and heat inactivation of both viruses resulted in dropping of the titre by 5 log10 in 120 minutes and complete virus inactivation at 60 176;C degree respectively. Either of these viruses is found suitable to be used as vaccine seed for production of future orf vaccine to protect sheep in Saudi Arabia. The obtained results were compared with those reported elsewhere in the literature. Key words: characterization; orf viruses; seeds; vaccine.
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