A Novel Design of Continuous Culture for In Vitro Formation of Gallstones by Salmonella Typhi

(Sahira I.H. Al-Sanjary and Amera M.M. Al-Rawi)

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Abstract

The current research focused on detecting the role of Salmonella typhi (S. typhi) in the formation of gallbladder stones in the laboratory following isolation and diagnosis of S. typhi from bile samples of patients suffering from gallstone. Locally and for the first time, a novel continuous culture was designed, and Brilliant Green Bile Broth (BGBB) was used by adding 60% cholesterol and 20% Calcium Carbonate (CaCO3) to form the gallstone nucleation. The continuous culture was inoculated with 1% S. typhi then incubated in optimal conditions for 20 days. After 14 days of incubation, results revealed the formation of spherical aggregations with various sizes in the test flask compared to the control flask. Moreover, an increase in the size of the stone formed was observed after 20 days of incubation. The morphology of cholesterol and Calcium Carbonate crystals were studied using light, fluorescent and scanning electron microscopes, and the functional groups were diagnosed using the Fourier transform infrared spectrometry (FTIR) technique.


KEYWORDS
Biliary microbiota, model Bile, polysaccharide production

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References

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