Anti-Fungal Activity of Thymoquinone and Amphotericine B Against Aspergillus Niger

(Abdul Rahman Mohammed Al Qurashi - Naim A)

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Abstract

Activity of Nigella sativa oil, ether extract and some of its active principles have been reported in the literature against a number of bacteria and Candida albicans. In the present study the effect of thymoquinone, an active principle of N. sativa and amphotericine B was determined against Aspergillus niger ATCC 16404. The organism was grown on dermasel agar containing 0.062, 0.125, 0.25, 0.5, 1.0 & 2.0 mg/ml of thymoquinone and dermasel agar alone as a control. There was 16.7, 36.2, 47.3, 67.8, 90.6 & 100% inhibition of growth of Aspergillus niger with these concentrations after 96 hours of incubation. Growth on the control plate after 96 hours was considered as 100%. Similarly there was 52.3, 65.1, 76.7, 81.6, 84.7, 85.6, 90.7, 92 and 93.8% inhibition of growth with 0.007, 0.015, 0.031, 0.062, 0.125, 0.25, 0.5, 1.0 and 2.0mg/ml of amphotericin B.
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