Micrografting Compatibility Between Two Almond Cultivars and Four Wild Species

(Wafaa Mohamed Koaym , Mohamed Abdul Razzaq Battha and Mhasen Mohamed Taher Twaklna )

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Abstract

Micropropagation and micrografting techniques are important methods used to obtain high quality plants. This research aimed to investigate the effect of wild almond species (Prunus communis, P. orientalis, P. korschinskii, and P. arabica) on the success of micrografting almond cultivars (Prunus dulcis cvs. Shami Furk and Dafadii)and determine  which combination of growth regulators lead to the highest rate of multiplication in  micrografted cultivars. The shoot tips were grafted onto the rooted rootstocks by inverted T-budding. The results indicated that Murashige and Skoog (MS) medium, supplemented with 1 mg/l benzyl adenine (BA), 0.1 mg/l indole-3-butyric acid (IBA) and 0.2mg/l gibberellic acid (GA3), achieved the highest shoot multiplication with an average of 5.31 and 3.67 shoots per explant and an average of 6.23cm and 4.98cm shoot length in cultivars Shami Furk and Dafadii, respectively. The highest grafting success rates were 80% and 74.26% obtained from Shami Furk/P. arabica and Dafadii/P. arabica combinations, respectively, while the lowest success rate was 50.63% with the Dafadii/P. orientalis combination. The liquid MS medium supplemented with 0.5 mg/l BA + 0.1 mg/l IBA achieved the highest micrografting success and scion shoot length. This research can be used to improve almond cultivation.

KEYWORDS
Almond, compatibility, growth regulators, microscions, rootstocks, tissue culture

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References

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