Scientific Journal Of King Faisal University
Basic and Applied Sciences

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Scientific Journal of King Faisal University / Basic and Applied Sciences

Opportunistic Bacteria Isolated from Trypauchen Vagina Fish that Infected with Protozoan from Iraq Marine Water

(Nadia Ali Al-Shammari )

Abstract

Opportunistic bacteria are microorganisms that have been isolated from a host suffering from stress as a result of parasitic infestation. In this study, two bacterial species, namely Aeromonas hydrophila and Rhizobium radiobacter, were isolated from Trypauchen vagina fish that were infected with Microsporidian parasite cysts from Iraqi marine waters. The infection were mass implanted in the internal and external skeletal muscles and under the gill cover. Many symptoms of bacterial infection were observed in the fish, including flatulence with haemorrhagic fluids, skin  disscolouration blackening, separation of crusts, and deformities in the area. The two bacterial species were characterised morphologically and identified using Vitek II system (Biomerieux - USA). To control infection, antibiotic susceptibility was performed using six antibiotics: nalidixic acid, nitrofurantoin, gentamicin, streptomycin, tetracycline and amoxicillin, both bacteria showed high antibiotic resistance. Identification of opportunistic bacteria has a pivotal role in preventing stress factors in fish, treating infections and alleviating their impact on other aquatic organisms.

KEYWORDS
Aeromonas hydrophila, microsporidian parasite, rhizobium radiobacter

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References

Al-Maleky, G.M. and Hanafi, R.A. (2013). Survey of aeromonas hydrophila in three marine fish species from North West Arabian Gulf, Iraq. Journal of THI-QAR Science, 3(4), 3–8.
Al-Shemmari, N.A. (2017). Isolation and Diagnosis of Bacteria Associated With Some Disease Infections in Some Fishes in Basra Governorate, Iraq. Master’s Dissertation, College of Agriculture, University of Basra, Iraq.
Austin, B. and Austin, D.A. (2007). Bacterial Fish Pathogens: Disease of Farmed and Wild Fish. Chester: Springer. DOI: 10.1007/978-1-4020-6069-4.
Bhagirathan, U. Panda, S.K. Meenakumari, B. Madhu, V.R. and Vaghela, D.T. (2012). Effects of bottom trawling on the ecological integrity of macrobenthos of Veraval, Gujarat. Journal Marine Biological Association of India, 54(1), 5–12. DOI. 10.6024/jmbai.2012.54.1.01528- 0
Carpenter, K.E. Krupp, F. Jones, D.A. and Zajonz, U. (1997). FAO Species Identification Guide for Fishery Purposes. The Living Marine Resources of Kuwait, Eastern Saudi Arabia, Bahrain, Qatar, and the United Arab Emirates. Rome: FAO Publications. Available at: http://www.fao.org/docrep/010/v8729e/v8729e00.htm (accessed on 11/11/2019).
Cheesbrough, M. (2000). District Laboratory Practice in Tropical Countries. Parts 2. Cambridge: Cambridge University Press.
CLSI, Clinical Laboratory Standards Institute (2006). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard (9th Ed). Wayne, PA: Clinical Laboratory Standards Institute.
Darby, C. Jennifer, W.H., Nafisa, G. and Stanley, F. (2002). Caenorhabditis elegans: Plague bacteria biofilm blocks food intake. Journal Nature, 16(4), 243–4. DOI: 10.1038/417243a.
Derome, N. Gauthier, J. Boutin, B. and Llewellyn, M. (2016). Bacterial opportunistic pathogens of fish. In: Hurst, C.J. (Ed.) The Rasputin Effect: When Commensals and Symbionts Become Parasitic., Switzerland: Springer. DOI: 10.1007/978-3-319-28170-4_4
Dias, M.K.R., Sampaio, L.S., Proietti, A.A., Junior, E.T.O., Yoshioka, D. P., Rodrigues, A.F.R., Rodriguez, R.A., Ribeiro, F.S., Faria, R.O.A., Ozório, M. and Tavares, D. (2016). Lethal dose and clinical signs of aeromonas hydrophila in arapaima gigas (Arapaimidae). Journal Veterinary Microbiology, 188(4), 12–5. DOI: 10.1016/j.sjbs.2017.10.019.  
Donia, G.R., Hafez, A. and Wassif, M. (2018). Studies on some heavy metals and bacterial pollutants in tilapia fish of El Salam Canal, Northern Sinai, Egypt. Egyptian Journal of Aquatic Biology & Fisheries Zoology, 21(4), 67–84. DOI: 10.13140/RG.2.2.18437.81121.
Fehrenbacher, K., Huckaba, T., Yang, H.C., Boldogh, I. and Pon, L. (2003). Actin comet tails, endosomes and endosymbionts. Journal of Experimental Biology, 12(2), 977–1984. DOI: 10.1242/jeb.00240 
Guo , H., Stefanie, P., Glaeser, I.A., Jafargholi, I., Hossein, H.P. and Karl-Heinz, K. (2017). The abundance of endofungal bacterium rhizobium radiobacter (syn. agrobacterium tumefaciens) increases in its fungal host piriformospora indica during the tripartite sebacinalean symbiosis with higher plants. Frontiers in Microbiology, 8(629), n/a. DOI: 10.3389/fmicb.2017.00629.
Hazen, T.C., Flierman, C.B., Hirsch, R.P. and Esch, G.W. (1978).  Prevalence and distribution of Aeromonas hydrophila in the United States. Appl. Journal of Environ. Microbiol., 36(5), 731–8. 
Hung, Y.T., Lee, T.A. and Huang, L.J. (2009). Clinical characteristics of patients with Acinetobacterjunii infection. Journal of Microbiology Immunology and Infection, 42(1), 47–53. DOI.10.1007/s10096-012-1622-x .
Jaafar, R.S. (2019). Bioremediation of lead and cadmium and the strive role of Pediococcus pentosaceus probiotic. Iraqi Journal of Veterinary Sciences, 34(1), 51–7.
Janda, J. and Abbot. S. (2010). The genus Aeromonas: Taxonomy, pathogenicity, and infection. Journal of Clinical Microbiology, 23(1), 35–73. DOI: 10.1128/CMR.00039-09.
Knochel, S. (1989). Effect of temperature on hemolysin production in Aeromonas spp. isolated from warm and cold environments. International Journal of Food Microbiology, 9(3), 225–35. DOI: 10.1016/0168-1605(89)90092-5.
Lom, J. and Dykova, I. (2005). Microsporidian xenomas in fish seen in wider perspective. Journal of Folia Parasitologica, 52(1–2), 69–81. DOI. 10.14411/fp.2005.010.  
Manuel, J.B., Omar, A.C., Gabriela, I., Cecilia, H., Leda, I.P., Luis, U.G. and Graciela, C. (2019). Horizontal gene transfer and its association with antibiotic resistance in the Genus Aeromonas spp. Journal of Microorganisms, 7(9), 363. DOI: 10.3390/microorganisms7090363 .
Marta, R., Damaso, C., Silva, J.E. and Almeida, M. (2011) Peritonitis due to Rhizobium radiobacter. Journal of Einstein (Sao Paulo), 9(3), 389–90. DOI:10.1590/S1679-45082011RC2025.
Noga, E. J. (2010). Fish Disease, Diagnosis and Treatment. USA: Wiley-Blackwell. ISBN: 978-0-813-80697-6 .
Oliver, K.M., Russell, J.A., Moran, N.A. and Hunter, M.S. (2003). Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Journal Proc Natl Acad Sci USA, 18(4), 1803–7. DOI:10.1073/pnas.0335320100. 
Olumide, A.O. and Ahmad, A. (2015). Antibiotic resistance profiling and phenotyping of aeromonas species isolated from aquatic sources. Saudi Journal Biol Sci., 24(1), 65–70. DOI: 10.1016/j.sjbs.2015.09.016 .
Putra, A.R., Effendi, M.H, Koesdarto, S., Suwarno, S. W., Tyasningsih, W. and Estoepangestie, A.T. (2019). Detection of the extended spectrum β-lactamase produced by escherichia coli from dairy by using the vitek-2 method in Tulungagung Regency, Indonesia. Iraqi Journal of Veterinary Sciences, 34(1), 203–7. DOI: 10.33899/ijvs.2019.125707.1134 .
Romero, J., Feijoó, C.G. and Navarrete, P. (2012). Antibiotics in Aquaculture – Use, Abuse and Alternatives, Health and Environment in Aquaculture. Available at: https://www.semanticscholar.org/paper/Antibiotics-in-Aquaculture (accessed on 11/11/2019).
Sreedharan, K.R. and Philip, I.S.B. (2012). Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: A possible threat to public health. Brazilian Journal of Microbiol, 43(2), 754–65. DOI: 10.1590/S1517-83822012000200040
Stratav, A. and Olumide, O. (2015). Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. Journal of Infection and Public Health, 9(5), 535–44. DOI: 10.1016/j.jiph.2015.10.006