Molecular Studies on the Effects of Auxins and Cytokinins on Vinca Rosae, Grown by Plant Tissue Culture

(Sumayah I. Alsanie, Mohey Elden K. Beharry and Mona S. Alahmadi)

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Abstract

Vinca Rosea (Apocynacea) is an important medicinal plant. In this study, the tissue culture propagation of vinca, using explants from stems and leaves, was investigated. Single and combined treatments of different phytohormones were added to Murashige and Skoog (MS) media. The genetic variation between cell lines derived from these explants was evaluated using protein electrophoresis and the determination of nucleic acids concentrations. The analysis of protein patterns showed that the number of protein bands in control was 16; indeed, in all treatments that number ranged between 4–33bands, regardless of explant origin. The polymorphism ratio was high in stem explants treatments through the incubation period in comparison to the control, while that ratio decreased in leaves explants treatments. The treatment with Naphthalene acetic acid (NAA) hormone was the closest to the control sample. It was noticed that the level of protein concentration resulting from stems explants culture was notably higher than those recorded in leaves explants. Measurements of nucleic acids concentrations showed that the highest significant concentration for DNA and RNA resulted from stems explants treatments. In conclusion, it is recommended to use stem explants and NAA hormone to achieve a generation of plants similar to the origin when using tissue culture technique.

KEYWORDS
genetic variations, protein patterns, polymorphism ratio, vinca rosea tissue culture

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References

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