المجلة العلمية لجامعة الملك فيصل
العلوم الأساسية والتطبيقية
المجلة العلمية لجامعة الملك فيصل / العلوم الأساسية والتطبيقية
مقارنة النشاط المضاد للفطريات للثيموكينون كمادة فعالة في الحبة السوداء (النجيللا ساتيفا) الامفوتيرسين بي ضد ميكروب الفيوسريم سولاني
(نعيم أخطر، عمر محمد الأكلبي، ص)
الملخص
The activity of thymoquinone, an active principle of Nigella sativa, and amphotericin B was compared against a clinical isolate of Fusarium solani. The organism was isolated from a nail lesion of an immunocompetent adult male in routine fungal culture on dermasel agar. For susceptibility testing the organism was grown on two sets of dermasel agar containing, 1.0, 0.5, 0.25, 0.125, 0.062 & 0.031 mg of thymoquinone and amphotericin B/ml. It was grown on dermasel agar alone as control. The growth on 10th day of inoculation was recorded as % inhibition taking growth of control as 100%. There was 0, 2.0, 3.0, 18.3, 59.3, and 100% inhibition of growth of Fusarium solani with 0.031, 0.062, 0.125, 0.25, 0.5, 1.0 mg thymoquinone /ml on 10th day of incubation. At similar concentrations of amphotericin B, there was 6.2, 24.5, 31.2, 39.7, 54.4 and 72.4% inhibition of growth. There was no complete inhibition of growth at any concentration of the drug. The study shows that at higher concentrations thymoquinone, giving a steap dose-effect relationship, more effectively inhibited the growth of a clinical isolate of Fusarium solani as compared to amphotericin B which gave shallow dose-effect relationship. However, amphotericin B was better at lower concentrations.
المراجع